Crocetin Protects Ultraviolet A-Induced Oxidative Stress

These findings suggest that crocetin its observed protective effects against UV-A induced skin damage by reducing reactive oxygen species production and cell apoptosis.

Crocetin Protects Ultraviolet A-Induced Oxidative Stress

Crocetin protects ultraviolet A-induced oxidative stress and cell death in skin in vitro and in vivo

Crocetin, the aglycone of crocin, is a carotenoid found in fruits of gardenia (Gardeina jasminoides Ellis) and saffron (Crocus sativus L.). We investigated the protective effects of crocetin against ultraviolet-A (UV-A)-induced skin damage and explored the underlying mechanism. Human skin-derived fibroblasts cells (NB1-RGB) were damaged by exposure to UV-A irradiation (10J/cm(2)). Crocetin protected these cells against cell death and reduced the production of reactive oxygen species induced by UV-A irradiation. Crocetin treatment also suppressed induction of caspase-3 activation by UV-A irradiation. The effects of crocetin against oxidative stress were also examined by imaging of Keap1-dependent oxidative stress detector (OKD) mice. UV-A irradiation upregulated oxidative stress in the OKD mice skin, while crocetin administration (100mg/kg, p.o.) ameliorated this oxidative stress. Crocetin administration also decreased lipid peroxidation in the skin. These findings suggest that crocetin its observed protective effects against UV-A induced skin damage by reducing reactive oxygen species production and cell apoptosis.

Takuya Ohba 1, Mitsue Ishisaka 1, Saori Tsujii 1, Kazuhiro Tsuruma 1, Masamitsu Shimazawa 1, Koya Kubo 2, Naofumi Umigai 2, Takao Iwawaki 3, Hideaki Hara 4  Eur J Pharmacol. 2016 Oct 15;789:244-253.