Preeclampsia is characterized by a large number of neutrophils

1) Neutrophils from patients with PE are more “metabolically active”; 2) This basal change in the level of metabolic activation is enhanced in response to bacterial products.

Preeclampsia is characterized by a large number of neutrophils

Preeclampsia is characterized by a large number of neutrophils displaying a high amplitude of NAD(P)H metabolic oscillations: A link between intravascular inflammation, endothelial cell dysfunction and preeclampsia

Objective
Neutrophils have been implicated in the pathophysiology of preeclampsia (PE) through their role in intravascular inflammation and endothelial cell dysfunction. The production of reactive oxygen metabolites (ROMs) is key to neutrophil function. ROM production is mediated by the actions of the enzyme NAD(P)H oxidase. This enzyme “oscillates” and the amplitude and frequency of such oscillations are linked to the production of ROMs. This study was conducted to characterize the pattern of NAD(P)H oscillations in PE.

Study design
This cross-sectional study included normal pregnant women (n=73) and patients with PE (n=40). NAD(P)H autofluorescence was used to assess the metabolic state of the cells and tetramethylrosamine fluorescence to characterize H202 release. The number of cells displaying high or low amplitude NAD(P)H oscillations and high or low oscillatory frequencies per 100 leukocytes were recorded. A p<0.05 was considered significant.

Results
1) Patients with PE had significantly more neutrophils with high amplitude and low frequency NAD(P)H oscillations than normal pregnant women; 2) LPS stimulation of neutrophils from normal pregnant women and PE lead to a higher increase in NAD(P)H oscillation frequency in PE than in normal pregnancy.

1) Neutrophils from patients with PE are more “metabolically active”; 2) This basal change in the level of metabolic activation is enhanced in response to bacterial products.

Jimmy Espinoza Andrei Kindzelski Juan Pedro Kusanovic Tinnakorn Chaiworapongsa Sonia Hassan Bo Hyun Yoon Howard Petty Roberto Romero  American Journal of Obstetrics and Gynecology Volume 195, Issue 6, Supplement, December 2006, Page S147